---
title: RNA extraction from mammalian cells — protocol v1
project: research-lab-template
tags:
  - protocol
  - rna
  - wet-lab
  - safety
date: 2026-04-07
---

# RNA extraction from mammalian cells — protocol v1

**Status:** Active | **Owner:** Lab manager | **Revision:** v1 (template)

## Scope

Total RNA from **1e6–5e6 adherent or suspension mammalian cells** using column-based cleanup. For BSL-2 material, complete institutional training before use.

## Materials

RNA kit (log vendor lot), 70/100% ethanol, optional on-column **DNase** per kit insert, **β-mercaptoethanol** (hood only; toxic).

## Steps (summary)

Harvest → PBS wash → pellet; lyse with **fresh** reducer (vortex 10s, 5 min RT); 70% ethanol bind; column washes per insert; optional DNase for qPCR-grade RNA; elute **30 µL** water; A260/280 and RIN if sequencing.

## Safety

- β-mercaptoethanol: use in chemical hood; avoid skin contact.
- Phenol-chloroform variants (if substituted): only in designated hood with training.

## QC & revisions

Target A260/280 ~1.9–2.1; store at −80°C if not used the same day. **v1:** initial Knowtation template structure.